Journal: Nature Communications

Article Title: A vascularized liver microphysiological system captures key features of hepatic insulin resistance and monocyte infiltration

doi: 10.1038/s41467-025-68031-6

Figure Lengend Snippet: A Schematic of culture platform and seeding strategy, beginning with multicellular hepatic spheroid generation in alginate microwells for 2 days, followed by harvesting and co-encapsulation of spheroids, endothelial cells, and supporting fibroblasts inside of a fibrin hydrogel within polydimethylsiloxane (PDMS) microfluidic devices. Created in BioRender. Tevonian, E. ( https://BioRender.com/o38c467 ). B Self-organized vascular network formation after 6 days in devices. Maximum intensity projection of live image taken at 10x magnification shows GFP-HUVEC networks (green) interacting with hepatocytes labeled with CellTracker Deep Red (magenta). Inset (yellow box) shows a magnified view, with arrows indicating regions where networks are in close proximity with spheroids. Scale bars = 200 µm. This experiment with CellTracker-labeled spheroids was repeated independently seven times with similar results. C Live image taken on Day 4 after device seeding shows RFP-HUVECs (false-colored cyan) migrating out of spheroids to connect with GFP-HUVECs (green), with hepatocytes labeled with CellTracker Deep Red (magenta). Scale bar = 200 µm. This experiment with RFP-HUVECs was repeated independently nine times with similar results. ( D ) Representative maximum intensity projection image of an immunostained device after 14 days of culture. Arginase-1 (magenta) indicates hepatocytes, CD31 (green) indicates HUVECs, and CD163 (white) indicates Kupffer cells. Scale bars = 200 µm. This staining was repeated for devices from three unique primary liver cell donors with similar results.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; Angioproteomie, cAP-0001) and normal human lung fibroblasts (NHLFs; Lonza, CC-2512) were cultured in flasks using VascuLife VEGF Endothelial Medium Complete Kit (VascuLife; LifeLine, LL-0003) and FibroLife S2 Fibroblast Medium Complete Kit (FibroLife; Lifeline, LL-0011), respectively.

Techniques: Encapsulation, Labeling, Staining

Journal: Microbial Cell Factories

Article Title: Recombinant L-asparaginase from Stenotrophomonas maltophilia : a promising low-immunogenic anticancer agent

doi: 10.1186/s12934-025-02856-0

Figure Lengend Snippet: Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

Article Snippet: Human leukemia cancer cell line K-562, human hepatocellular carcinoma cell line HepG-2, and normal human lung fibroblast cell line MRC-5 were purchased from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Activity Assay, Recombinant, Produced, Standard Deviation